The bacterial cellulose (BC) has been reported widely. Although there are many methods to modify BC, such as the oxidized BC, which is biodegradable and can be used as wound dressing. However, the nanostructure of BC makes it difficult to be oxidized. Importantly, high oxidation degree makes the content of aldehyde high, which make the cell biocompatibility poor. Herein, we fabricated a novel bio-composite based on microporous oxidized BC (MOBC) and in-situ grafted with Arg. The micropores can increase the contact area between BC and oxidizing agent and the reaction between MOBC and Arg, which will enhance the biocompatibility. The roughness and surface energy of MOBC/68.68%Arg are 1.5 and 1.16 times than that of BC respectively. We applied a microfluidic chip to evaluate the cell migration. Comparing with BC, MOBC/Arg promoted proliferation, migration and expression of Collagen-I of fibroblasts and endothelial cells. It prospects the MOBC/Arg can be used as wound dressing.