A human skull was discovered at the 16th-century drainage channel of market district ruins, one of the busiest streets in the capital of Joseon kingdom. By anthropological examination, we noticed the cut mark at the right occipital part of the cranium. Judging from the wound property, it might have been caused by a strong strike using a sharp-edged weapon. As no periosteal reaction or healing signs were observed at the cut mark, he might have died shortly after the skull wound was made. We speculated that this might have been of a civilian or soldier victim who died in a battle or the decapitated head of prisoner. This is the first report about the discovery of the skull damaged by sharp-edged weapon at the archaeological sites in the capital city of Joseon Kingdom.