Septins are involved at the early stages of macroautophagy in <i>S. cerevisiae</i>.
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Abstract | :
Autophagy is a conserved cellular degradation pathway wherein double-membrane vesicle called autophagosomes capture long-lived proteins and damaged or superfluous organelles and deliver them to the lysosome for degradation. Septins are conserved GTP-binding proteins involved in many cellular processes, including phagocytosis and autophagy of intracellular bacteria, but no role in general autophagy was known. In budding yeast, septins polymerize into ring-shaped arrays of filaments required for cytokinesis. In an unbiased genetic screen and in subsequent targeted analysis, we found autophagy defects in septin mutants, and co-localized septins in rings at the pre-autophagosomal structure (PAS) and on autophagosomes where they physically interact with the autophagy proteins Atg8 and Atg9. Pre-assembled septin complexes relocalized to the PAS upon autophagy induction. Septin-mutant cells contained fewer autophagocytic structures, even when autophagosome degradation was blocked, and a mutation (atg1Δ) blocking PAS maturation, but not initial PAS assembly, decreased septin localization to the PAS. Our findings support a role for septins in the early stages of budding yeast autophagy, during autophagosome formation. |
Year of Publication | :
2018
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Journal | :
Journal of cell science
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Date Published | :
2018
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ISSN Number | :
0021-9533
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URL | :
http://jcs.biologists.org/cgi/pmidlookup?view=long&pmid=29361537
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DOI | :
10.1242/jcs.209098
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Short Title | :
J Cell Sci
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