Detection of carbonylated proteins in two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis separations.
| Author | |
|---|---|
| Abstract | :
Protein carbonyls are an index of protein oxidation which, in turn, reflects the interplay of oxidative stress and degradation of oxidatively modified proteins. Protein carbonyls are increased in brain proteins in aging and age-related neurodegenerative disorders, including Alzheimer's disease. In this chapter, we outline methods to detect protein carbonyls following two dimensional-based separation of brain proteins. |
| Year of Publication | :
0
|
| Journal | :
Methods in molecular biology (Clifton, N.J.)
|
| Volume | :
476
|
| Number of Pages | :
153-63
|
| Date Published | :
2008
|
| ISSN Number | :
1064-3745
|
| URL | :
https://dx.doi.org/10.1007/978-1-59745-129-1_11
|
| DOI | :
10.1007/978-1-59745-129-1_11
|
| Short Title | :
Methods Mol Biol
|
| Download citation |