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Tubulin dynamics during the cytoplasmic cohesiveness cycle in artificially activated sea urchin eggs.

Author
Abstract
:

Sedimentation studies and [3H]colchicine-binding assays have demonstrated a relationship between the cytoplasmic cohesiveness cycles and the changes in tubulin organization in Paracentrotus lividus eggs activated by 2.5 mM procaine. The same amount of tubulin (20-25% of the total egg tubulin) is involved in these cyclic process and appears to undergo polymerization and depolymerization cycles. Electron microscopy studies reveal that the microtubules formed during these cytoplasmic cohesiveness cycles are under a particulate form which is sedimentable at low speed. Activation experiments carried out in the presence of cytochalasin B (CB) show that the increase in the cytoplasmic cohesiveness is highly reduced while tubulin polymerization and depolymerization cycles and pronuclear centration are not affected. Although tubulin or actin polymerization can be independently triggered in procaine-activated eggs, the increase in cytoplasmic cohesiveness requires the polymerization of both proteins. However, the cytoplasmic cohesiveness cycles appear to be regulated by tubulin polymerization and depolymerization cycles.

Year of Publication
:
1983
Journal
:
Experimental cell research
Volume
:
149
Issue
:
2
Number of Pages
:
409-18
ISSN Number
:
0014-4827
URL
:
https://linkinghub.elsevier.com/retrieve/pii/0014-4827(83)90353-1
DOI
:
10.1016/0014-4827(83)90353-1
Short Title
:
Exp Cell Res
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