This study evaluated the measurement and construct validity of the Eating Disorder Examination Questionnaire Short Form (EDE-QS) in a transgender and gender diverse sample. Participants who self-identified as transgender and gender diverse ( = 71) completed self-report measures of demographics, gender-related experiences, and disordered eating/body image. Analyses comprised evaluation of EDE-QS internal consistency, convergent validity, and specificity for disordered eating. The EDE-QS demonstrated strong internal consistency in the full sample, in participants with and without suspected eating disorders, and in each gender identity group; and correlated significantly with indices of disordered eating and body image. The full scale and each item significantly differentiated between participants with and without suspected eating disorders. This study provides initial evidence for good measurement and construct validity of the EDE-QS as applied to transgender and gender diverse individuals. Findings offer the EDE-QS as a promising, brief tool for screening and/or population-based research related to disordered eating in this high-risk, yet underserved population. (PsycInfo Database Record (c) 2021 APA, all rights reserved).