Purpose: To investigate the effect of Colloidal Platinum Nanoparticles (CPN) on the bond strength between dentin and 4-META/MMA-TBB resin using different concentrations of CPN. Materials and Methods: Twenty-five extracted human third molars were stored in 0.5% chloramine T. The occlusal dentin slices were prepared by grinding occlusal surfaces of each tooth and polishing with 600-grit silicon carbide paper under running water. One control and four experimental groups (2 specimens per group) were used as follows: a) dentin surfaces treated with 10-3 solution, followed by rinsing with water and subsequently an acrylic rod bonded with hand-mixed 4META/MMA-TBB resin (Super-Bond C&B, Sun Medical) (control); b) dentin surfaces treated with 10-3 etching solution, followed by rinsing with water and application of CPN (100% or 10%) as a primer solution for 60 s and rinsed with water for 20 s, then an acrylic rod bonded with Super-Bond C&B(Etch-CPN [100% or 10%]); c) dentin surfaces treated with CPN (100% or 10%) for 60 s, rinsed with water for 20 s, followed by application of 10-3 solution, then an acrylic rod bonded with Super-Bond C&B (CPN-Etch [100% or 10%]). After storage in 37°C water, specimens were sectioned into beams (cross-sectional area: 1 mm2) for microtensile bond strength testing at a crosshead speed of 1mm/min. The data were analyzed using the Games-Howell method (p < 0.05; n = 15). Results: Etch-CPN (100), CPN-Etch(100) and CPN-Etch (10) showed significantly higher bond strengths compared to the control. When using 10% CPN, the highest bond strength was demonstrated. The bond strength of 4META/MMA-TBB resin was approximately doubled by CPN application. Conclusion: The results of this study showed that higher bond strengths are obtained when treating dentin with a lower concentration of CPN. Further evaluation to optimize conditions such as the application time and rinsing time are required.